TAG-IN Design

Example Input

Users may input Ensembl Transcript IDs or Custom sequences of choice

• Ensembl Transcript ID for GRCh38 or GRCm38 Ensembl builds - for example ENST00000229307
• Batch processing of Ensembl Transcript IDs - for example: ENST00000229307, ENST00000330949
• Custom sequence with strand orientation and stop codon position - for example AGCTAGAGCTAGCGACTAG...

For optimal use please utilise well annotated transcripts only.

Example Output

TAG-IN results are displayed in the following manner:

Transcript information and Dowloads

Transcript info:

Basic transcript info supplied for each query:

• Transcript start/stop, CDS orientation, stop position, and chromosome as per Ensembl database

Downloads:

Output for each job submission is in the form of two CSV files.

• Download: sgRNA and ssDNA list - sgRNA and ssDNA details for a given transcript submission including scoring and metrics.
• Download: Verbose Off-target Details- verbose details for all off-targets found.

Interactive sgRNA layout

Layout:

Each sgRNA around the stop codon is drawn and colour coded based on summation of efficiency, specificity and distance from stop.

• Query transcript is detailed in blue with the CDS and 3UTR separated by the stop codon.
• SgRNA are positioned along the transcript with either + or - direction.
• Only the top 20 sgRNA are displayed.

Interactivity:

• On mouse over the summation score and position of each sgRNA is given.
• On click the following sgRNA/Offtarget/ssDNA tables are filtered.

sgRNA/Off-target/ssDNA data tables

• Where an sgRNA acts within the intron/exon it will be prefixed with a *.
• Data tables are displayed for each group of sequences: sgRNA/Off targets/ssDNA
• Upon select of a chosen sgRNA the subsequent off target and ssDNA table will filter accordingly
• Filters can be cleared using the clear button.