Hello and welcome to TAG-IN...
A CRISPR sgRNA/ssDNA donor design tool for knock-in tag experiments - Developed by the Pollard lab.
TAG-IN is a sgRNA/ssDNA design tool aimed at optimising CRISPR knock-in tag experimentation. It provides a complete approach by considering both on and off-target sgRNA scoring models, as well as providing a novel ssDNA design function. TAG-IN provides a flexible selection of knock-in tags and a choice between both Human and Mouse genomes.
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"Dewari, P., et al. (2018). An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein delivery. (In preparation)"
A number of further options are due to be implemented including:
- 5 prime tagging
- Enumerative Off-target searching as per user choice (up to 6MM)
- Further user defined tags as required